Gene Expression Analysis of IgM+CD27+ B Cells in HCV-Infected Individuals with Mixed Cryoglobulinemia

ABSTRACT: Analysis of IgM+CD27+ B cells in individuals with hepatitis C virus (HCV)-associated mixed cryoglobulinemia (MC). We have previously shown that HCV+MC+ individuals have clonal expansions of IgM+ memory B cells. This study aims to characterize differentially expressed genes in peripheral IgM+CD27+ B cells in HCV RNA+MC+ individuals, compared to HCV RNA+MC- subjects and HCV RNA- healthy controls. IgM+ B cells were isolated form donor PBMCs by immunomagnetic negative selection, and the CD27+ subset was further purified by positive selection. 2ng RNA was isolated from cells and amplified using the Nugen WT-Ovation Amplification Kit. cDNA was synthesized, biotin labeled, and hybridized to Illumina human v2 microarrays. Data was collected in BeadStudio and exported to GeneSpring for analysis. Ra

ORGANISM(S): Homo sapiens

SUBMITTER: Edgar Charles 

PROVIDER: E-GEOD-18084 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress