Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profile from field-grown drought-stressed cotton

ABSTRACT: Drought-responsive genes were identified in leaf tissues of the cotton Acala 15-1799, a cotton genotype derived in the Southwest USA. Most of the identified genes such as the heat shock proteins have been described as responsive to drought and heat in other plant species. Microarray analysis was used to identify drought-responsive genes that might confer this cotton genotype with an improved tolerance to drought stress. Leaves from cotton plants watered or exposed to drought stress were used for RNA extraction and generarion of cRNA probes for hybridization of Affymetrix microarrays. Microarray technology was used to identify drought-responsive genes in cotton to assist in molecular breeding program. The supplementary file 'GSE18253_differentially_expressed.txt' lists the differentially expressed genes.

ORGANISM(S): Gossypium hirsutum

SUBMITTER: Jinfa Zhang

PROVIDER: E-GEOD-18253 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Systems responses of mature leaves from 4 reference cultivars of a larger collection of European potato cultivars (Solanum tuberosum L.) are investigated by metabolome profiling and RNA-Sequencing. The chosen reference cultivars, Milva, Alegria, Desiree, and Saturna, vary in ascending order in regard to drought tolerance. Systems analyses are based on 3 independent field trials and 3 paralleled greenhouse trials. Robust responses across all cultivars and conditions to natural seasonal drought stress comprise proline, raffinose, galactinol, arabitol, arabinonic acid, chlorogenic acid, and 102 transcripts which consist to a high proportion of heat shock proteins and genes with signaling or regulatory functions, such as a homolog of abscisic acid receptor PYL4. Constitutive differences of the tolerant cultivars, Desiree and Saturna, compared to the sensitive cultivars include arbutin (hydroquinone-beta-D-glucopyranoside), octopamine (p-hydroxyphenylethanolamine), ribitol and 248 differential transcripts. Many of these transcripts are disease related, receptor kinases, or regulatory genes, for example a homolog of the Arabidopsis FOUR LIPS MYB-regulator of stomatal cell proliferation. Functional enrichment analyses imply that heat stress is a major acclimation component of potato leaves to agronomical relevant drought stress. Enhanced leaf heat stress is a result of drought caused by loss of transpiration cooling. This effect and CO2-limitation are the main dilemmas of drought- or ABA-induced stomatal closure. Constitutive differences between tolerant and sensitive cultivars indicate partially synergistic interactions of drought and biotic stress responses. We suggest that drought tolerance of the potato reference cultivars may be caused by general resistance mechanisms which are part of previously selected pathogen tolerance. Transcriptome profiling by RNA-sequencing of 48 leaf samples from 4 potato cultivars grown under control or drought stress conditions in 6 independent experiments

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Gene expression profile from field-grown drought-stressed cotton

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