Nonsense Mediated mRNA decay mutes the splicing defects of spliceosome component mutations

ABSTRACT: The role of many splicing factors in pre-mRNA splicing and the involvement of these factors in the processing of specific transcripts have often been defined through the analysis of loss of function mutants in vivo. Here we show that inactivating the nonsense mediated mRNA decay (NMD) results in an enhancement of splicing phenotypes associated with several splicing factors mutations. Tiling microarrays showed that inactivation of the NMD factor Upf1p in the prp17Δ and prp18Δ mutant strains reveals a larger spectrum of splicing defects than what is observed in the single mutants, including new transcripts previously shown unaffected by Prp17p or Prp18p inactivation. In addition, inactivation of Upf1 in the prp22-1 mutant enhances the unspliced precursor accumulation phenotype of several s

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Matteo Pellegrini 

PROVIDER: E-GEOD-18288 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress