MicroRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Vitis vinifera

ABSTRACT: Vitis vinifera RNA degradome Isolated polyadenylated RNA from total RNA extracts of Vitis vinifera leaves, were ligated to 5'-adapter that include san MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were purified from gel and to a 3'- dsDNA adapter and PCR amplified. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

ORGANISM(S): Vitis vinifera

SUBMITTER: Simon Moxon 

PROVIDER: E-GEOD-18406 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress