Microarray analysis of Chlorella Virus PBCV-1 Transcription during the viral lifecycle

ABSTRACT: Paramecium bursaria chlorella virus (PBCV-1), a member of the family Phycodnaviridae, is a large dsDNA, plaque-forming virus that infects the unicellular green alga Chlorella NC64A. The 331 kb PBCV-1 genome is predicted to encode 365 proteins and 11 tRNAs. To follow global transcription during PBCV-1 replication, a microarray containing 50-mer probes to the PBCV-1 365 protein-encoding genes (CDS) was constructed. Competitive hybridization experiments were conducted employing cDNA from poly A-containing RNA obtained from cells at seven time points after virus infection. The results led to the following conclusions: i) the PBCV-1 replication cycle is temporally programmed and regulated; ii) 360 (99%) of the arrayed PBCV-1 CDSs are expressed at some time in the virus life cycle in the laborat

ORGANISM(S): Paramecium bursaria Chlorella virus 1

SUBMITTER: Giane Yanai-Balser 

PROVIDER: E-GEOD-18421 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress