FUR- mutant vs. WT

ABSTRACT: Ferric uptake regulator (Fur) is the major regulator of iron acquisition in Escherichia coli and other bacteria. In the present study, the role of Fur in anaerobic S. enterica serovar Typhimurium was determined by transcriptome analysis, reporter assays, and enzymatic assays. In anaerobic ?fur, 298 genes were differentially expressed. In general, Fur repressed genes required for iron acquisition/storage, metabolism, electron transport, oxidative/nitrosative stress, and modulators of virulence. There were 73 genes whose expression required Fur, eleven of which contain a putative Fur box 5? of the gene. Transcription of sodA was >9-fold higher in ?fur but there was no corresponding increase in the activity of SodA. Anaerobic cultures of the fur mutant and the WT were used to inoculate two s

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium

SUBMITTER: Ryan Fink 

PROVIDER: E-GEOD-18441 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress