Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

10 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Wild Type 11168 versus LuxS01 Mutant Cultured in MHB and MEM- Î± media

ABSTRACT: Transcriptional profiling of Campylobacter jejuni NCTC 11168 wild type versus LuxS01 mutant strain grown in a minimal culture medium (MEM- Î± ) versus complex culture medium (MHB). Two-condition experiment, the parent versus the mutant 11168 strains were grown in 2 types of culture medium for comparison. Control microarrays included comparing wild type vs wild type in the same culture medium. 4 biological replicates for each test microarray and 2 biological replicates for each control microarray, independently grown and harvested.

ORGANISM(S): Campylobacter jejuni

SUBMITTER: Kathryn Holmes

PROVIDER: E-GEOD-18451 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Addition of Exogenous AI-2 to C. jejuni 11168 Wild Type and LuxS01.

Project description:Transcriptional profiling of Campylobacter jejuni NCTC 11168 wild type and LuxS01 mutant strains comparing the effects of exogenously added in vitro-produced autoinducer 2 (AI-2) versus a control buffer to both strains. Two-condition experiment, addition of exogenous AI-2 to both the parent and mutant 11168 strains. Control microarrays included comparing 11168 with AI-2 free buffer vs 11168 with AI-2-free buffer and mutant with AI-2 free buffer versus mutant with AI-2 free buffer used for analyses. 3 biological replicates for each, independently grown and harvested.

2010-06-21 | E-GEOD-18427 | biostudies-arrayexpress

AI-2 does not function as a quorum sensing molecule in Campylobacter jejuni during exponential growth in vitro

Project description:This SuperSeries is composed of the following subset Series: GSE18427: Addition of Exogenous AI-2 to C. jejuni 11168 Wild Type and LuxS01. GSE18451: Wild Type 11168 versus LuxS01 Mutant Cultured in MHB and MEM- ? media Refer to individual Series

2009-10-08 | E-GEOD-18455 | biostudies-arrayexpress

Wild Type 11168 versus LuxS01 Mutant Cultured in MHB and MEM- α media

Project description:Transcriptional profiling of Campylobacter jejuni NCTC 11168 wild type versus LuxS01 mutant strain grown in a minimal culture medium (MEM- α ) versus complex culture medium (MHB).

2009-10-08 | GSE18451 | GEO

CGH Analysis of C. jejuni isolates

Project description:We performed microarray CGH analysis of 104 neuropathogenic and enteritis only stains of C. jejuni. Keywords: Individual hybridizations, CGH We measured signal intensity for tester strain versus control strain Cj NCTC 11168 for 104 isolates of C. jejuni.

2007-09-30 | E-GEOD-3579 | biostudies-arrayexpress

dgpp-2 DGPP-regulated genes: Identification of diacylglycerol pyrophosphate-regulated genes in ABA signaling

Project description:rs05-06_dgpp - dgpp - 2. The specific plant phosphorylated form of phosphatidic acid (PA), diacyglycerol pyrophosphate (DGPP), was recently shown to be a second messenger in abscisic acid (ABA) signaling. The aim of the project is to identify among the set of ABA-regulated genes the ones also regulated by DGPP and/or PA. 4 dye-swap - CATMA arrays 5ml of 3 days-old suspension cells were incubated with ABA or DGPP 18:1 for 3hours under the conditions of culture. DGPP was emulsified by sonication for 1min 4 times at 4Â°C in 1ml of culture medium then added to 4ml of suspension cells. Cells were filtrated under vacuum, frozen in liquid nitrogen and RNA extracted. DGPP is from Avanti Polar Lipids.

2010-05-19 | E-GEOD-12938 | biostudies-arrayexpress

meiocytes-Transcriptomic of meiocytes.

Project description:ra05-04_meiocytes - meiocytes - What are the gene expressed during meiosis pathway in Arabidopsis thalina. - Comparison of meiocyte transcriptome versus leaf transcriptome. Keywords: total transcriptome knowledge 1 dye-swap - CATMA arrays

2010-05-15 | E-GEOD-13000 | biostudies-arrayexpress

nitric oxide-reducing microbial community metaproteomics

Project description:Here, we successfully used NO as the direct electron acceptor for the enrichment of a microbial community in a continuous bioreactor. The enrichment culture, mainly comprised of two new organisms from the Sterolibacteriaceae family, grew on NO reduction to N2 and formate oxidation, with virtually no accumulation of N2O. The microbial growth kinetics of the enrichment culture as well as its affinity for different N-oxides were determined. In parallel, using metagenomics, metatranscriptomics, and metaproteomics, the biochemical reactions underlying the growth of these microorganisms on NO were investigated. This study demonstrates that microorganisms thrive and can be enriched on NO, and presents new opportunities to study microbial growth on this highly energetic and climate-active molecule that may have been pivotal in the evolution of aerobic respiration.

2023-06-09 | PXD037586 | Pride

CGH analysis of Campylobacter jejuni isolates previously analyzed by Multi-Locus Sequence Typing

Project description:In this experiment, we hava analyzed a set of 45 C. jejuni strains by CGH and MLST in order to investigate whether the strain relationships inferred from the analysis of a small number of loci (MLST) reflect whole-genome gene conservation patterns observed by CGH. We performed microarray CGH on 45 strains previously characterized by MLST. Genomic DNA from each experimental strain was compared in a CGH experiment against the reference strain NCTC 11168. We measured signal intensity for each tester strain versus the control strain NCTC 11168. Values represent mean of replicate spots. Replicate arrays are not included.

2008-04-21 | E-GEOD-9919 | biostudies-arrayexpress

dgpp-DGPP regulated genes. Identification of diacylglycerol pyrophosphate regulated genes in ABA signaling.

Project description:rs05-06_dgpp - dgpp - The specific plant phosphorylated form of phosphatidic acid (PA), diacyglycerol pyrophosphate (DGPP) was recently shown to be a second messenger in abscisic acid (ABA) signaling. The aim of the project is to identify among the set of ABA-regulated genes the ones also regulated by DGPP and/or PA. - Five ml of 3 days-old suspension cells was incubated with ABA or lipids for 3 h under the conditions of culture. Lipids were emulsified by sonication for 1 mi, four times, at 4Â°C, in one ml of culture medium then added to 4 ml suspension cells. Cells were filtrated under vacuum, frozen in liquid nitrogen and RNA extracted. Dioleoyl PA and dioctanoyl PA are from Sigma, dioleoyl DGPP and dioctanoyl DGPP are from Avanti Polar Lipids. Keywords: treated vs untreated comparison 5 dye-swap - CATMA arrays

2010-05-19 | E-GEOD-13019 | biostudies-arrayexpress

Ozone exposure of tolerant and sensitive F2 genotypes of Populus

Project description:Three tolerant and three sensitive genotypes of the inbred QTL mapping population Family 331 were exposed to either ambient air or an acute ozone exposure of 200 ppb for 9 hrs and ozone response was profiled for each genotype by hybridising control against ozone-exposed samples per genotype. Keywords: stress response, genotype comparrison, ozone exposure Three replicates were grown per genotypes used and RNA was extracted from the fifth leaf below the first fully unfurled leaf. After RNA extraction, equal quantities of RNA from each replicate of a genotype per treatment were pooled to provide a single pool per treatment per genotype. Control and ozone-exposed sample per genotype were then hybridised. Hald of all arrays were hybridised with the control sample in the Cy3 channel and half with control in the Cy5 channel.

2010-06-20 | E-GEOD-10873 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data