ChIP-chip from HCT116 cells treated with MMS and LNCaP cells with ATF3

ABSTRACT: Maintenance of genetic integrity is essential for survival of all organisms. Activating transcription factor 3 (ATF3) is a member of the c-AMP response element binding (CREB)/ATF family of transcription factors, and is highly inducible by various stress conditions including DNA damage. However, downstream targets and molecular basis underlying pleiotropic effects of ATF3 on the cell fate have been largely unknown. To identify ATF3 targets in the human genome, we carried out chromatin immunoprecipitation-microarray (ChiP-on-chip) and knockdown-expression profiling analysis using two models where ATF3 was either transiently induced or constitutively expressed. We show that ATF3 binds to an unexpectedly large number of targets; 5,984 promoters in HCT116 cells treated with an alkylating agene

ORGANISM(S): Homo sapiens

SUBMITTER: yujiro tanaka 

PROVIDER: E-GEOD-18457 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress