Double fragmentation ChIP-seq provides single nucleotide resolution Tcf4 binding characteristics

ABSTRACT: ChIP-seq has become the method of choice for studying functional DNA-protein interactions on a genome wide scale. The method is based on co-immunoprecipitation of DNA binding proteins with formaldehyde cross-linked DNA, followed by deep sequencing of immunoprecipitated chromatin fragments, allowing for the identification of binding sites with high accuracy [1-5]. Traditionally, genome-wide tiling path microarrays were used for the detection of specifically immunoprecipitated DNA fragments, but current next-generation sequencers now generate sufficient data to assay multiple samples in a single sequencing run, making this method more time and cost effective compared to microarray-based approaches [2]. However, due to limitations in read length of next generation sequencing technologies (50-

ORGANISM(S): Homo sapiens

SUBMITTER: Michal Mokry 

PROVIDER: E-GEOD-18481 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress