Germination of Leafy Spurge (Euphorbia esula) Seeds under Constant and Alternating Temperature

ABSTRACT: Transcriptome changes were investigated for Euphorbia esula (leafy spurge) seeds with a focus on the effect of constant and diurnal fluctuating temperature on dormancy and germination. Leafy spurge seeds do not germinate when incubated for 21 days at 20°C constant temperatures, but nearly 30% germinate after 21 days under fluctuating temperatures 20:30°C (16:8 h). Incubation at 20°C for 21 followed by 20:30°C resulted in approximately 63% germination in about 10 days. A cDNA microarray representing approximately 22,000 unique sequences was used to profile transcriptome changes. Labeled cDNA was prepared from total RNA using the Alexa Fluor cDNA labeling kit (Invitrogen, Carlsbad, CA) according to manufacture's protocols. Labeled cDNAs were hybridized to a custom made 23 K element microarra

ORGANISM(S): Euphorbia esula

SUBMITTER: Michael Foley 

PROVIDER: E-GEOD-18533 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress