Comparison of immature BMDC to BMDC after nkt cell modulation

ABSTRACT: Analyze the gene expression profile of BMDC before and after nkt cell modulation. Total RNA was extracted from CD11c cells with TRIzol Reagent, purified with RNeasy Kit (Qiagen) and subsequently amplificated using the one cycle target labeling kit according to the AffymetrixGeneChip® Expression Analysis Technical Manual (Affimetrix). Fragmented cRNAs were probed to AffymetrixGeneChip® Mouse Genome 430 2.0 arrays for 16 hours at 45°C in the AffymetrixHybridization Oven 640. The arrays were then washed and stained with streptavidin-phycoerythrin using the AffymetrixFluidic Station 450/250 and scanned using the AffymetrixGeneChip® Scanner 3000. The images were analyzed using GCOC® Software and comparison analyses carried out according to the instructions provided by Affymetrix. Each exp

ORGANISM(S): Mus musculus

SUBMITTER: Ester Badami 

PROVIDER: E-GEOD-18547 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress