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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Chromatin Profiling by Directly Sequencing Small Quantities of Immunoprecipitated DNA

ABSTRACT: Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties. However, current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is accurate, compatible with just 50 picograms of DNA and should thus facilitate charting chromatin maps from limited cell populations. Application of a single-molecule approach to directly sequence chromatin immunoprecipitated DNA of the CTCF DNA binding protein.

ORGANISM(S): Mus musculus

SUBMITTER: Alon Goren

PROVIDER: E-GEOD-18699 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA.

Goren Alon A Ozsolak Fatih F Shoresh Noam N Ku Manching M Adli Mazhar M Hart Chris C Gymrek Melissa M Zuk Or O Regev Aviv A Milos Patrice M PM Bernstein Bradley E BE

Nature methods 20091129 1

Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell p ...[more]

PMID: 19946276

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