M.ruminantium_B.proteoclasticus_coculture_vs_monocultures

ABSTRACT: This study was undertaken with two major goals in mind: i) To investigate the potential for interspecies hydrogen transfer between a hemicellulytic rumen bacterium (B. proteoclasticus) and a methanogenic achaea (M. ruminantium) Microscopic examination had previously shown B. proteoclasticus in co-culture with M. ruminantium rapidly formed cell to cell co-aggregates. ii) To examine the expression of genes involved in methanogenesis under more rumen-like conditions. Cells of M.ruminantium and B. proteoclasticus were grown in triplicate as mono- or co-cultures until a mid-exponential growth phase was reached. RNAs were extracted and purified. The relative quantities of RNAs contributed by each organism to the co-culture samples were determined by rt qPCR using genes previously shown to be con

ORGANISM(S): Butyrivibrio proteoclasticus B316

SUBMITTER: Carl Yeoman 

PROVIDER: E-GEOD-18716 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress