Physiology and genetic activity of Listeria monocytogenes during multi-hurdle stress induced cell death

ABSTRACT: The physiological basis of the hypothesis that temperature is the primary governing factor of bacterial cell inactivation under otherwise non-growth permissive conditions was investigated. Application of simultaneous low pH (pH 3.5) and low water activity (aw = 0.9; 2.5 M NaCl) conditions, applied to L. monocytogenes strains Scott A and FW03/0035, and increasing incubation temperature from 25°C up to 45°C resulted in increased permeability to ethidium homodimer-1 and corresponded to accelerated declines in esterase activity and ATP basal levels but did not result in autolysis. Triphasic survival curves were readily observable when sufficiently large cell populations were inactivated at 25°C and 35°C; indicative of a mixture of sensitive and resistant sub-populations. Enrichment-based r

ORGANISM(S): Listeria monocytogenes

SUBMITTER: John Bowman 

PROVIDER: E-GEOD-18796 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress