3D culture of AIDS-NHL cells influences gene expression related to B-cell development, proliferation and survival

ABSTRACT: Full title: Three-dimensional culture of AIDS-NHL cells influences gene expression related to B-cell development, proliferation and survival The AIDS-NHL-derived cell line, UMCL01-101, representing diffuse large B-cell lymphoma of immunoblastic morphology (AIDS-IBL), was grown in conventional, static suspension culture or three-dimensionally (3D) in the Rotating Wall Vessel (RWV) bioreactor. The objective was to assess the impact on gene expression of growth as a three-dimensional tissue assembly. Global gene expression analysis was performed on UMCL01-101 cells grown under either condition using Affymetrix microarray. UMCL01-101 cells were cultured in the Rotating Wall Vessel bioreactor to form 3D assemblies, or in conventional suspension culture, for 15 days. RNA was prepared from trip

ORGANISM(S): Homo sapiens

SUBMITTER: Kerstin Honer zu Bentrup 

PROVIDER: E-GEOD-18826 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress