PML-RARa/RXR alters the epigenetic landscape in Acute Promyelocytic Leukemia

ABSTRACT: Many different molecular mechanisms have been suggested to be associated with PML-RAR dependent transformation of haematopoietic progenitors. Here, we investigated the role of PML-RAR and RXR in the organization of epigenetic structures at a genome-wide level. We identified 2722 high confidence PML-RAR binding sites in the leukemic model cell line NB4 and found PML-RAR colocalization with RXR to the vast majority of these binding regions. Importantly, these results could be corroborated and extended in primary blast cells of two APL patients. Through genome-wide epigenetic studies we show that treatment with pharmacological doses of all-trans retinoic acid ATRA induces global alterations in active H3 acetylation and repressive H3K27me3, H3K9me3 and DNA methylation chromatin modifications.

ORGANISM(S): Homo sapiens

SUBMITTER: H.G. Stunnenberg 

PROVIDER: E-GEOD-18886 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress