Transcription profiling of rat duodenal samples at different developmental stages and the effects of iron-deficiency
Ontology highlight
ABSTRACT:
ORGANISM(S): Rattus norvegicus
SUBMITTER: James Forrest Collins
PROVIDER: E-GEOD-1892 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Identification of differentially expressed genes in response to dietary iron deprivation in rat duodenum.
Collins James F JF Franck Christina A CA Kowdley Kris V KV Ghishan Fayez K FK
American journal of physiology. Gastrointestinal and liver physiology 20050106 5
We sought to identify novel genes involved in intestinal iron absorption by inducing iron deficiency in rats during postnatal development from the suckling period through adulthood. We then performed comparative gene chip analyses (RAE230A and RAE230B chips; Affymetrix) with cRNA derived from duodenal mucosa. Real-time PCR was used to confirm changes in gene expression. Genes encoding the apical iron transport-related proteins [divalent metal transporter 1 (DMT1) and duodenal cytochrome b] were ...[more]
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