Tonicity iduced changes in gene expression in IMCD cells and the effect of Cyclosporin A
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ABSTRACT: Cyclosporin A induces expression of proapoptotic factors when cells are challenged by increased tonicity 18 Samples were analyzed. We performed the following pairwise comparison using BRB Array Tools: 300 vs. 600_24; 300 vs. 600_24_CSA; 600 vs. 900_24; 600 vs. 900_24_CSA
Project description:Tonicity induced massive changes in gene expression. Already the acute challange for 3 h affected significantly the expression of thousands of genes. The overall differences in gene expression were hightes between cells cultivated at 300 compared to cells cultivated at 900 mosmol/kg 30 Samples were analyzed
Project description:Calu-3 cells are human lung cancer cells that serve as a model of respiratory epithelial cell model. Here, Calu-3 cells are used as a cell system for Middle East respiratory syndrome coronavirus and treated with Cyclosporin A To assess the effect of Cyclosporin A on the host cells, uninfected cells were treated with CsA or DMSO, RNA was extracted and compared against the DMSO treated control cells.
Project description:We analyzed the gene expression at the onset of hair growth induced by cyclosporin A (CsA), a well-known hair growth inducer, with DNA microarray, and unveiled the step-by-step progression of hair growth. KEYWORDS: Cyclosporin A, Cyclosporine A, Ciclosporin A
Project description:The transcriptomics changes induced in the human liver cell line HepG2 by Cyclosporin A after treatment for 12h, 24h, 48h and 72h The study investigated differential gene expression in HepG2 cell line mRNA following 12, 24, 48 and 72 hours of exposure to Cyclosporin A and solvent. Three biological replicates per compound/solvent. In total 36 arrays.