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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression profile of ypr045cM-bM-^HM-^F mutant

ABSTRACT: We report a new protein complex with a role in transcription elongation that is formed by Ypr045c (Thp3) and the Csn12 component of the COP9-signalosome. Thp3-Csn12 is recruited to transcribed genes. Their mutations suppress the gene expression defects of mutants of the THO complex involved in mRNP biogenesis and export and show defects in mRNA accumulation. In vivo transcription elongation impairment of thp3M-bM-^HM-^F mutants is shown by reduction of RNAPII recruitment throughout an active gene and in transcript run on analysis performed in G-less systems. This new complex establishes a novel link between transcription and mRNA processing. Tthree repeats of the wild type control and three of the ypr045cM-bM-^HM-^F mutant (BY4741 background).

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Sonia Jimeno

PROVIDER: E-GEOD-18994 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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New suppressors of THO mutations identify Thp3 (Ypr045c)-Csn12 as a protein complex involved in transcription elongation.

Jimeno Sonia S Tous Cristina C García-Rubio María L ML Ranes Michael M González-Aguilera Cristina C Marín Antonio A Aguilera Andrés A

Molecular and cellular biology 20101213 4

Formation of a ribonucleoprotein particle (mRNP) competent for export requires the coupling of transcription with mRNA processing and RNA export. A key link between these processes is provided by the THO complex. To progress in our understanding of this coupling, we have performed a search for suppressors of the transcription defect caused by the hpr1Δ mutation. This has permitted us to identify mutations in the genes for the RNA polymerase II mediator component Med10, the Sch9 protein kinase, a ...[more]

PMID: 21149575

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