Analysis of DNA strand-specific differential expression with high density tiling microarrays

ABSTRACT: DNA microarray technology allows the analysis of genome structure and dynamics at genome-wide scale. Expression microarrays (EMA) contain probes for annotated open reading frames (ORF) and are widely used for the analysis of differential gene expression. By contrast, tiling microarrays (TMA) have a much higher probe density and provide unbiased genome-wide coverage. The purpose of this study was to develop a protocol that would take advantage of the high resolution of TMAs for quantitative measurement of DNA strand-specific differential expression of annotated and non-annotated transcripts. We extensively filtered probes present in Affymetrix Genechip Yeast Genome 2.0 expression and GeneChip S. pombe 1.0FR tiling microarrays to generate custom Chip Description Files (CDF) in order to compa

ORGANISM(S): Schizosaccharomyces pombe 972h-

SUBMITTER: Luis Quintales 

PROVIDER: E-GEOD-19020 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress