Gene Expression Analysis of Sox17-induced Mouse Embryonic Stem Cells

ABSTRACT: To determine the effect of Sox17 overexpression in mouse embryonic stem (ES) cells, we performed gain-of-function analysis by generating ES cell lines carrying a doxycycline inducible FLAG-tagged Sox17 transgene. We treated Sox17-inducible ES cells with doxycycline, collected RNA and performed genome-wide transcriptional analysis. We found that genes invovled in adhesion function and basement membrane establishment were transcriptionally upregulated in ES cells upon induction of Sox17. We also observed downregulation in the transcription of genes involved in pathways known to be functionally important for ES cell pluripotency and self-renewal. However, Sox17 expression was not sufficient to rapidly down-regulate Sox2, Nanog, and Oct4. Two independent doxycycline inducible Sox17-overexpress

ORGANISM(S): Mus musculus

SUBMITTER: Kathy Niakan 

PROVIDER: E-GEOD-19028 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress