EYA4 is a non-small cell lung cancer tumor suppressor located in the susceptibility locus on chromosome 6q

ABSTRACT: This study was undertaken to identify novel tumour suppressor genes in lung adenocarcinoma. EYA4, located at 6q23.2 was identified as a frequently lost and hypermethylated gene in the analyzed samples. EYA4 is underexpressed in addition to being deleted and hypermethylated. Control of EYA4 expression by DNA methylation was assessed using 5'-azacytidine. The role of EYA4 in apoptosis was assessed using a stable knock down of the gene which was assessed for apoptotis using FACS and qPCR. Methylation profiling: 30 adenocarcinoma samples and 30 patient-matched non-malignant lung samples are included. Non-malignant samples were used as references to identify hypermethylated probes. For each normal/tumor pair, the sample numbers are paired but offset by one with the tumor being the lower o

ORGANISM(S): Homo sapiens

SUBMITTER: Ian Wilson 

PROVIDER: E-GEOD-19034 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress