Comparative transcriptional profiling of Limbal Epithelial Crypts with rest of the ocular surface epithelial regions.

ABSTRACT: Recently we had discovered a solid cord like structure at the limbus of human eyes, termed as the Limbal Epithelial Crypt (LEC). It arises from the undersurface of the interpalisade rete ridges and extends towards the conjunctiva over the conjunctival stroma. Anatomical and immunohistochemical studies have shown it to be potentially a Stem Cell Niche. To confirm this hypothesis we conducted comparative gene expression profile of LEC with pathway and Geneontology studies in comparison with other ocular surface epithelial regions such as cornea, limbus, LEC stroma and conjunctiva. Frozen tissue blocks of corneoscleral buttons dissected from cadaver eyes were cryosectioned. These tissue sections from different ocular surface regions were laser microdissected. Extracted RNA was amplified & h

ORGANISM(S): Homo sapiens

SUBMITTER: Patrick Tighe 

PROVIDER: E-GEOD-19035 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress