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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Pichia angusta cells glucose- vs methanol-grown

ABSTRACT: Transcriptional profiling of Pichia angusta NCYC 495 leu1.1 cells growing exponentially on mineral medium containing 0.5% glucose versus a shift for 2 hours to mineral medium containing 0.5% methanol. Two-conditions experiment, 4 individual glucose-grown cultures were shifted at OD660nm 2.3 to fresh mineral medium OD 0.2 containing 0.5% methanol for 2 hours. Transcriptional profiling was performed using total RNA from the glucose-grown cells at the moment of the shift versus cells adapted to methanol for 2 hours.

ORGANISM(S): Pichia angusta NCYC 495 leu1.1

SUBMITTER: Ida van der Klei

PROVIDER: E-GEOD-19036 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Adaptation of Hansenula polymorpha to methanol: a transcriptome analysis.

van Zutphen Tim T Baerends Richard J S RJ Susanna Kim A KA de Jong Anne A Kuipers Oscar P OP Veenhuis Marten M van der Klei Ida J IJ

BMC genomics 20100104

<h4>Background</h4>Methylotrophic yeast species (e.g. Hansenula polymorpha, Pichia pastoris) can grow on methanol as sole source of carbon and energy. These organisms are important cell factories for the production of recombinant proteins, but are also used in fundamental research as model organisms to study peroxisome biology. During exponential growth on glucose, cells of H. polymorpha typically contain a single, small peroxisome that is redundant for growth while on methanol multiple, enlarge ...[more]

PMID: 20044946

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Pichia angusta cells glucose- vs methanol-grown

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