Hepatocytes obtained from mouse GPSCs are functional and show similar expression profiles to fetal primary hepatocytes

ABSTRACT: Germline cell-derived pluripotent stem cells (GPSCs) are similar to embryonic stem (ES) cells in that they can proliferate intensively and differentiate into a variety of cell types, including cardiomyocytes and neurons. In this report, mouse GPSCs were induced to differentiate into hepatocytes with very high efficiency, and demonstrated, for the first time, to be functional. These hepatocytes were characterised at cellular and molecular levels. The GPSC-derived hepatocytes not only expressed hepatic markers, but were also metabolically active as shown by albumin and haptoglobin secretion, urea synthesis, glycogen storage and indocyanine green uptake. Previous studies have revealed some inherent differences in gene expression between undifferentiated mouse ES cells and GPSCs. We wanted t

ORGANISM(S): Mus musculus

SUBMITTER: Sharmila Fagoonee 

PROVIDER: E-GEOD-19044 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress