Degradome sequencing reveals endogenous small RNA targets in rice (Oryza sativa L. ssp. indica)
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ABSTRACT: MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) regulate gene expression in eukaryotes. Plant miRNAs modulate their targets mainly via messenger RNA (mRNA) cleavage. Small RNA targets have been extensively investigated in Arabidopsis using computational prediction, experimental validation, and degradome sequencing. However, small RNA targets are largely unknown in rice (Oryza sativa). Here, we report global identification of small RNA targets using high throughput degradome sequencing in the rice indica cultivar 93-11 (Oryza sativa L. ssp. indica). 177 transcripts targeted by total of 87 unique miRNAs were identified. Of targets for the conserved miRNAs between Arabidopsis and rice, transcription factors comprise around 70% (58 in 82), indicating that these miRNAs act as masters of
ORGANISM(S): Oryza sativa Indica Group
SUBMITTER: xiaofeng cao
PROVIDER: E-GEOD-19050 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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