Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression based analysis of CroV infection of Cafeteria roenbergensis

ABSTRACT: In this study, total RNA was extracted from cultures of Cafeteria infected with the Cafeteria roenbergensis virus BV-PW1 (CroV) and hybridised to a CroV microarray in order to determine the temporal expression profile during infection. A straightforward measure of whether each spot was on or off was determined as the infection progressed starting at 0 h post infection (control), then 1, 2, 3, 6, 12, 24, 48 and 72 h pi. Oligonucleotides 50-70 bp in length were designed for 452 predicted CroV ORFs and printed onto amino silane treated glass slides.

ORGANISM(S): Cafeteria roenbergensis virus BV-PW1

SUBMITTER: Michael Allen

PROVIDER: E-GEOD-19051 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Giant virus with a remarkable complement of genes infects marine zooplankton.

Fischer Matthias G MG Allen Michael J MJ Wilson William H WH Suttle Curtis A CA

Proceedings of the National Academy of Sciences of the United States of America 20101025 45

As major consumers of heterotrophic bacteria and phytoplankton, microzooplankton are a critical link in aquatic foodwebs. Here, we show that a major marine microflagellate grazer is infected by a giant virus, Cafeteria roenbergensis virus (CroV), which has the largest genome of any described marine virus (≈730 kb of double-stranded DNA). The central 618-kb coding part of this AT-rich genome contains 544 predicted protein-coding genes; putative early and late promoter motifs have been detected an ...[more]

PMID: 20974979

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Dataset Information

Gene expression based analysis of CroV infection of Cafeteria roenbergensis

Publications

Giant virus with a remarkable complement of genes infects marine zooplankton.

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