Transcription profiling of activated mixed glial (microglia) and monocyte-derived macrophages

ABSTRACT: Since macrophage activation can now be studied at a global level using modern microarray and proteomic analyses, discovery of novel macrophage activation genes is inevitable and important for understanding HIV-associated dementia (HAD). We isolated two different types of primary human macrophages, microglia and monocyte-derived macrophages (MDM) from brain tissue and whole blood respectively. The microarray analysis of differentially regulated macrophage activation genes reported here supports our previous assertions that the mixed glia (MIX) cultured in starvation conditions (DMEM alone) are a non-activated, or quiescent, tissue culture model for studying macrophage activation in the brain. Transcript levels from these quiescent cultures provided a background level of gene expression, and

ORGANISM(S): Homo sapiens

SUBMITTER: Andrew Albright 

PROVIDER: E-GEOD-1910 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress