Transcription profiling of S. cerevisiaeAir-liquid interfacial biofilm vs planktonic S. cerevisiae cells

ABSTRACT: Goal was to identify yeast genes whose expression changed as a function of the shift from growth in bulk culture to growth in an air-liquid interfacial biofilm. Experiment Overall Design: Cells were grown 24 h at 30 C in YEPD to a density of about 500,000,000 cells/ml. At harvest, sugar was found to be depleted as measured by an enzymatic dip stick (Diastic, Bayer). Cells were pelleted and washed twice in sterile distilled water by centrifugation and then diluted 10-fold into 100 ml of Flor medium (YNB + 4% ethanol + leucine + histidine + uracil) in triplicate 250 ml beakers. Cultures were then grown statically in Flor medium at 27 C. Within a few hours following inoculation, the bulk liquid appeared to be clear, no biofilm was evident by visual observation, and a layer of settled cells wa

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Alan Bakalinsky 

PROVIDER: E-GEOD-19156 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress