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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Xenopus egg small RNA associated with Y12 antibody

ABSTRACT: We examined in Xenopus tropicalis eggs piRNAs that are associated with Y12 antibody, which binds symmetrically methylated arginines that are present on diverse Piwi proteins. The goal of this experiment is to more deeply characterize the piRNA pool from adult Xenopus extracts, using the Illumina platform. Sequencing of a cDNA library from small RNAs from the Y12 immunoprecipitate

ORGANISM(S): Xenopus (Silurana) tropicalis

SUBMITTER: Nicolas Robine

PROVIDER: E-GEOD-19173 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A broadly conserved pathway generates 3'UTR-directed primary piRNAs.

Robine Nicolas N Lau Nelson C NC Balla Sudha S Jin Zhigang Z Okamura Katsutomo K Kuramochi-Miyagawa Satomi S Blower Michael D MD Lai Eric C EC

Current biology : CB 20091201 24

<h4>Background</h4>Piwi-interacting RNAs (piRNAs) are approximately 24-30 nucleotide regulatory RNAs that are abundant in animal gonads and early embryos. The best-characterized piRNAs mediate a conserved pathway that restricts transposable elements, and these frequently engage a "ping-pong" amplification loop. Certain stages of mammalian testis also accumulate abundant piRNAs of unknown function, which derive from noncoding RNAs that are depleted in transposable element content and do not engag ...[more]

PMID: 20022248

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