Transcription profiling of mouse Primary cerebellar granule cell precursors (GCPs) cultures were prepared from P7 Bmi-1-/- or wild type to investigate the role of Bmi1 in Shh-mediated shift in gene expression levels

ABSTRACT: We investigated the role of Bmi1 in Shh-mediated shift in gene expression levels Experiment Overall Design: Primary cerebellar granule cell precursors (GCPs) cultures were prepared from P7 Bmi-1-/- or wild type mice as described before {Messer, 1977 #810}. Cells were grown in Dulbecco MEM high Glucose, supplemented with 10% FCS glutamine, 20 mM KCl and penicillin–streptomycin for 24 hours. Shh (R&D Systems) if appropriate was added for 24 hours to give at final concentration of 3 mg/ml. Experiment Overall Design: We performed 3 biological replicates for both WT and Bmi-1-/- cultures, and 2 biological replicates for both WT and Bmi-1-/- cultures, treated with Shh. Experiment Overall Design: Total RNA was isolated using RNeasy Mini Kit (Qiagen) and reverse-transcribed into double-strande

ORGANISM(S): Mus musculus

SUBMITTER: Tatiana Subkhankulova 

PROVIDER: E-GEOD-19176 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress