Analysis of germination and outgrowth of sorbic acid-stressed Bacillus cereus ATCC 14579 spores.

ABSTRACT: Sorbic acid (SA) is widely used as a preservative, but the effect of SA on spore germination and outgrowth has gained limited attention up to now. Therefore, the effect of sorbic acid on germination of spores of B. cereus strain ATCC 14579 was analyzed both at phenotype and transcriptome level. Spore germination and outgrowth was assessed at pH 5.5 without and with 0.75, 1.5 and 3.0mM (final concentrations) undissociated sorbic acid (HSA). This resulted in distinct HSA concentration-dependent phenotypes, varying from delays in germination and outgrowth to complete blockage of germination at 3.0mM HSA. The phenotypes reflecting different stages in the germination process could be confirmed using flow cytometry and could be recognized at transcriptome level by distinct expression profiles. I

ORGANISM(S): Bacillus cereus ATCC 14579

SUBMITTER: Clint van Melis 

PROVIDER: E-GEOD-19186 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress