Depleting cytosolic cysteine compromises the antioxidant capacity of the cytosol in Arabidopsis thaliana

ABSTRACT: Plant cells contain different O-acetylserine(thiol)lyase (OASTL) enzymes involved in Cys biosynthesis and located in different subcellular compartments. These enzymes are made up of a complex variety of isoforms resulting in different subcellular Cys pools. To unravel the contribution of cytosolic Cys to plant metabolism, we characterized the knockout oas-a1.1 and osa-a1.2 mutants, deficient in the most abundant cytosolic OASTL isoform in Arabidposis thaliana. Total intracellular Cys and glutathione concentrations were reduced, and the glutathione redox state was shifted in favour of its oxidized form. Interestingly, the capability of the mutants to chelate heavy metals did not differ from that of the wild type, but the mutants have an enhanced sensitivity to Cd. With the aim of establishi

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Cecilia Gotor 

PROVIDER: E-GEOD-19245 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress