Gene expression in circulating mononuclear cells after exposure to ultrafine carbon particles

ABSTRACT: Peripheral blood samples were collected before (0 hour) and at 24 hours after exposure from healthy subjects who participated in previous controlled exposures to ultrafine carbon particles (UFP, 50 microg/m3) or filtered air (FA)(n = 3 each). The exposure time was 2 hours. RNA from mononuclear cell fraction (>85% lymphocytes) was extracted, amplified and hybridized to Affymetrix HU133 plus 2 microarrays. We used microarray to explore significantly altered genes after ultrafine carbon particle exposure. Each subject was exposed to filtered air or ultrafine carbon particles. Two peripheral blood samples (pre- and post-exposure) were taken. Mononuclear cells were isolated for gene expression analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Yuh-Chin Huang 

PROVIDER: E-GEOD-19317 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress