Monovalent and unpoised status of most genes in undifferentiated cell-enriched Drosophila testis

ABSTRACT: We use male gonads isolated from a Drosophila strain that allows us to obtain enough cells at their primitive status as the starting material to study the endogenous chromatin structure of undifferentiated cells using ChIP-seq. We integrate the ChIP-seq data with RNA-seq data that measures the transcriptome in a digital manner. Our genome-wide analyses indicate that the majority of differentiation genes in undifferentiated cells lack an active chromatin mark and paused Pol II; instead, they are associated with either the repressive H3K27me3 mark or no detectable mark. In order to address the possibility that distinct techniques are responsible for such a difference, we also use the Drosophila S2 cells to perform ChIP-seq and RNA-seq and compare the results directly with published work usin

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Dustin Schones 

PROVIDER: E-GEOD-19325 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress