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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human fibroblasts grown on TCPS plates, Collagen-GAG 2 D plate and Collagen-GAG 3D mesh

ABSTRACT: All mRNA was isolated after 8 hours of culture time in each of three culture conditions. (1) TCPS Plate, (2) Collagen-GAG 2 dimensional coated plate and (3) collagen-GAG three dimensional mesh.

ORGANISM(S): Homo sapiens

SUBMITTER: Catherine Klapperich

PROVIDER: E-GEOD-1937 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Fibroblast remodeling activity at two- and three-dimensional collagen-glycosaminoglycan interfaces.

Jaworski Justyn J Klapperich Catherine M CM

Biomaterials 20060418 23

Previously we demonstrated that high throughput gene expression experiments can yield novel information about how cells respond to a collagen-glycosaminoglycan (GAG) three-dimensional culture environment. The goal of the current study was to determine which of these differences result from culture in a three-dimensional construct versus those caused simply by the presence of the collagen-GAG biomaterial. To make this distinction, cells were cultured both in collagen-GAG scaffolds fabricated usin ...[more]

PMID: 16620959

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Collagen-GAG Biocompatibility Tests

Project description:All mRNA was isolated after 8 hours of culture time in each of three culture conditions. (1) TCPS Plate, (2) Collagen-GAG 2 dimensional coated plate and (3) collagen-GAG three dimensional mesh. Keywords: ordered

2004-11-09 | GSE1937 | GEO

IMR-90 cells: Collagen Mesh Time Course

Project description:A series of chips with some repeated measurments. Each chip represents a pool of 4 collagen/chondroitin sulfate tissue engineering scaffold meshes seeded with 1 x 10^6 IMR-90 Human Fibroblasts. mRNA was isolated at 30 minutes, 1, 2, 4, 8, 12, 24, 48, and 72 hours after seeding. Keywords = tissue engineering mesh, collagen, chondroitin sulfate, biomaterials design Keywords: time-course

2003-09-10 | GSE409 | GEO

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Project description:Collagen-GAG Biocompatibility Tests

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Transcription profiling of human fibroblasts grown in petri dishes vs. attached to a tissue engineering scaffold sampled at 7 timepoints.

Project description:A goal of tissue engineering is to produce a scaffold material that will guide cells to differentiate and regenerate functional replacement tissue at the site of injury. Little is known about how cells respond on a molecular level to tissue engineering scaffold materials. In this work we used oligonucleotide microarrays to interrogate gene expression profiles associated with cell-biomaterial interactions. We seeded collagen-glycosaminoglycan meshes, a widely used tissue engineering scaffold material, with human IMR-90 fibroblasts and compared transcript levels with control cells grown on tissue culture polystyrene. Genes involved in cell signaling, extracellular matrix remodeling, inflammation, angiogenesis and hypoxia were all activated in cells on the collagen-GAG mesh. Understanding the impact of a scaffold on attached cells will facilitate the design of improved tissue engineering materials.

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Project description:We performed a 3' RACE of a novel HIV RNA TAR-gag in order to determine the sequence of the RNA at the 3' end. Our data had shown that TAR-gag was potentially a noncoding RNA and our hypothesis was that TAR-gag ended somewhere prior to the end of the gag region of the HIV genome. The 3' RACE experiment showed that TAR-gag actually consists of four different RNA clusters, the longest of which ends at 615 bases from the transcription start site; this is in the middle of the p17 region of the gag gene. In addition, we sequenced all host RNAs in the EVs.

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