Transcriptional profiling of E. coli after addition of peroxynitrite or hydrogen peroxide to aerobically growing cells

ABSTRACT: Peroxynitrite is formed in macrophages by the diffusion-limited reaction of superoxide and nitric oxide. This highly reactive species is capable of causing both oxidative and nitrosative stress in Escherichia coli. Previous studies have focused on the reactions of peroxynitrite with specific proteins or the effects of peroxynitrite on the growth and viability of whole cells. This work shows for the first time the transcriptomic response of E. coli to peroxynitrite, highlighting specific areas targeted by the stress. Upregulation of the cysteine biosynthesis pathway and subsequent identification of an increase in S-nitrosothiol levels suggests S-nitrosylation as a consequence of peroxynitrite exposure. Genes involved in the assembly / repair of iron-sulfur clusters also show enhanced transc

ORGANISM(S): Escherichia coli

SUBMITTER: Robert Poole 

PROVIDER: E-GEOD-19370 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress