KLF2-induced actin shear fibers control both alignment to flow and JNK signaling in vascular endothelium

ABSTRACT: The shear stress-induced transcription factor Krüppel-like factor 2 (KLF2) confers anti-inflammatory properties to endothelial cells through inhibition of activator protein 1, presumably by interfering with MAPK cascades. To gain insight into the regulation of these cascades by KLF2, we used antibody arrays in combination with time-course mRNA micro-array analysis. No gross changes in MAPKs were detected, rather phosphorylation of actin cytoskeleton-associated proteins, including Focal Adhesion Kinase, was markedly repressed by KLF2. Furthermore, we demonstrate that KLF2-mediated inhibition of Jun NH2-terminal kinase (JNK) and its downstream targets ATF2/c-Jun is dependent on the cytoskeleton. Specifically, KLF2 directs the formation of typical short basal actin filaments, we term shear f

ORGANISM(S): Homo sapiens

SUBMITTER: Anton Horrevoets 

PROVIDER: E-GEOD-19412 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress