Comprehensive Characterization of 3D Models for Prostate Cancer Growth and Invasion in Laminin-rich Extracellular Matrix

ABSTRACT: Full title: Comprehensive Characterization of Three-Dimensional Models for Prostate Cancer Growth and Invasion in Laminin-rich Extracellular Matrix Prostate Cancer (PrCa) cells undergo acinar morphogenesis and spheroid formation in three-dimensional (3D) culture, supported by laminin-rich extracellular matrix (lrECM, Matrigel). We developed miniaturized 3D model systems that facilitate investigation of morphogenesis and invasion of normal and PrCa cell lines in lrECM. Primary and non-transformed cell lines formed round structures with strong cell-cell contacts and epithelial polarization, lumen and a complete basal lamina (BL). In contrast, most PrCa cell lines formed either defective, “mass” spheroids with incomplete BL, or invasive “stellate” structures. The bioinformatic analyses of gen

ORGANISM(S): Homo sapiens

SUBMITTER: Matthias Nees 

PROVIDER: E-GEOD-19426 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress