Profiling antibody responses to M. tuberculosis proteins in TB suspects’ sera using full-proteome microarrays.

ABSTRACT: Understanding the immune response to tuberculosis requires greater knowledge of humoral responses. To characterize antibody targets and the effect of disease parameters on target recognition, we developed a systems immunology approach that integrated detection of antibodies against the entire Mycobacterium tuberculosis proteome, bacterial metabolic and regulatory pathway information, and patient data. Probing ~4,000 M. tuberculosis proteins with sera from >500 suspected tuberculosis patients worldwide revealed that antibody responses recognized ~10% of the bacterial proteome. This result defines the immunoproteome of M. tuberculosis, which is rich in membrane-associated and extracellular proteins. Most serum reactivity during active tuberculosis focused onto ~0.5% of the proteome. Wit

ORGANISM(S): Homo sapiens

SUBMITTER: Shajo Kunnath-Velayudhan 

PROVIDER: E-GEOD-19433 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress