Activin/Nodal signalling controls divergent transcriptional networks in pluripotent and endoderm progenitors.

ABSTRACT: Activin/Nodal signalling is necessary to maintain pluripotency of human Embryonic Stem Cells (hESCs) and to induce their differentiation towards endoderm. However, the mechanisms by which Activin/Nodal signalling achieves these opposite functions remain unclear. To unravel these mechanisms, we examined the transcriptional network controlled in hESCs by Smad2 and Smad3 which represent the direct effectors of Activin/Nodal signalling. These analyses reveal that Smad2/3 participate in the control of the core transcriptional network characterising pluripotency which includes Oct-4, Nanog, FoxD3, Dppa4, Tert, Myc and UTF-1. In addition, similar experiments performed on endoderm cells confirm that a broad part of the transcriptional network directing differentiation is downstream of Smad2/3. The

ORGANISM(S): Homo sapiens

SUBMITTER: Matthew Trotter 

PROVIDER: E-GEOD-19461 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress