Understaning mechanisms underlying human gene expression variation with RNA sequencing

ABSTRACT: Understanding the genetic mechanisms underlying natural variation in gene expression is a central goal of both medical and evolutionary genetics, and studies of expression quantitative trait loci (eQTLs) have become an important tool for achieving this goal. While all eQTL studies to date have assayed mRNA levels using expression microarrays, recent advances in RNA sequencing enable the analysis of transcript variation at unprecedented resolution. We sequenced RNA from 69 lymphoblastoid cell lines (LCLs) derived from unrelated Nigerian individuals that have been extensively genotyped by the International HapMap Project. Pooling data from all individuals, we generated a map of the transcriptional landscape of these cells, identifying extensive use of unannotated polyadenylation sites and ov

ORGANISM(S): Homo sapiens

SUBMITTER: Joseph Pickrell 

PROVIDER: E-GEOD-19480 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress