Temporal patterns in the transcriptomic response of rainbow trout, Oncorhynchus mykiss, to crude oil

ABSTRACT: Time is often not characterized as a variable in ecotoxicogenomic studies. In this study, the temporal kinetics in gene expression were determined during exposure to crude oil and a subsequent recovery period. Juvenile rainbow trout, Oncorhynchus mykiss, were exposed for 96 hours to the water accomodated fractions of 0.4, 2 or 10 mg l-1 crude oil loadings. Following 96 h of exposure, fish were transferred to recovery tanks for 96 h. Gill and liver samples were collected after 24 and 96 h of exposure, and after 96 h of recovery for RNA extraction and microarray analysis. Fluorescently labeled cDNA was hybridized against matched controls, using salmonid cDNA arrays. Each exposure scenario generated unique patterns of altered gene expression. More genes responded to crude oil in the gil

ORGANISM(S): Oncorhynchus mykiss

SUBMITTER: Sharon Hook 

PROVIDER: E-GEOD-19489 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress