3CB-induced gene expression of Pseudomonas putida KT2440 in soil

ABSTRACT: To know whether the microarray technique could be used to detect bacterial gene expression in soil, large quantity of RNA was extracted from soil cultures of Pseudomonas putida KT2440 containing a chloroaromatic degrading plasmid at the presence or absence of the growth substrate, 3-chlorobenzoate (3CB). The quality and quantity of the extracted RNA were proper for a typical microarray analysis. Gene expression patterns of soil cultures were analyzed by DNA microarray using the extracted RNA. Among 5346 genes on the array, 5% and 4.5% of genes showed up- or down-regulation. Analysis done at the DAVID Bioinformatics Resources server suggested that the benzoate degradation via hydroxylation pathway had the most significant changes after treatment with 3CB. Expression of the 3CB degradation g

ORGANISM(S): Pseudomonas putida KT2440

SUBMITTER: yong wang 

PROVIDER: E-GEOD-19516 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress