Screening of genes involved in chromosome segregation in meiosis I

ABSTRACT: Prophase I of male meiosis involves dynamic chromosome segregation processes during early spermatogenesis, including synapsis, meiotic recombination, and cohesion. Genetic defects in genes participating in these processes consistently cause reproduction failure in mice. To identify candidate genes responsible for infertility in humans, we performed expression profiling of mouse spermatogenic cells undergoing meiotic prophase I. Cell fractions enriched in spermatogonia, leptotene/zygotene spermatocytes, or pachytene spermatocytes were separately isolated from mouse testes for RNA extraction. To minimize the contamination of other cell types, we fractionated the testicular cells undergoing the first round of spermatogenesis using Percoll gradient procedure. The cell fractions were character

ORGANISM(S): Mus musculus

SUBMITTER: Hiroshi Kogo 

PROVIDER: E-GEOD-19532 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress