Comparative ChIP-chip analysis of general transcription factor TFIIB and negative cofactor NC2 in human B cells

ABSTRACT: A comparative ChIP-chip analysis of TFIIB and NC2 in human B cells reveals that basal core promoter architectures control the equilibrium between NC2 and preinitiation complexes. We conducted a comparative ChIP-chip and gene expression analysis of TFIIB in human B cells and analyze associated core promoter architectures. TFIIB occupancy relates well to gene expression, with the vast majority of promoters being GC-rich and lacking defined core promoter elements. TATA consensus and TATA-like motifs but not the previously in vitro defined TFIIB recognition elements (BREs) are enriched in approximately 5% of the genes. Further insight was obtained by performing a parallel ChIP-chip analysis of the TFIIB antagonist NC2. The latter identifies a highly related target gene set. Nonetheless, subpop

ORGANISM(S): Homo sapiens

SUBMITTER: Thomas Albert 

PROVIDER: E-GEOD-19562 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress