Arp2/3 complex mutants show a pronounced lack of hyphal specific gene expression in Candida albicans

ABSTRACT: Candida albicans is a diploid fungal pathogen lacking a defined complete sexual cycle, and thus has been refractory to standard forward genetic analysis. Instead, transcription profiling and reverse genetic strategies based on Saccharomyces cerevisiae have typically been used to link genes to functions. To overcome restrictions inherent in such indirect approaches, we have investigated a forward genetic mutagenesis strategy based on the UAU1 technology. We screened 4700 random insertion mutants for defects in hyphal development, and linked two new genes (ARP2 and VPS52) to hyphal growth. Deleting ARP2 abolished hyphal formation, generated round and swollen yeast phase cells, disrupted cortical actin patches and blocked virulence in mice. The mutants also showed a global lack of induction o

ORGANISM(S): Candida albicans

SUBMITTER: Zully Leon 

PROVIDER: E-GEOD-19565 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress