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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from Arabipdosis msh1 recA3 double mutant under heat stress

ABSTRACT: In Arabidposis thaliana, the msh1 recA3 double mutant shows an extensive mitochondrial genome rearrangement and displays pronounced thermotolerance. We use microarray analysis to characterize genes and their response in the double mutant to a heat shock experiment in which plants were exposed to a temperature of 37 C for 2 hours. Wild type and double mutant plant were exposed to a temperature of 37 C for 2 hours and RNA samples were collected for the wild type and the double mutant before and after the heat shock.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jaime Davila

PROVIDER: E-GEOD-19603 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Extensive rearrangement of the Arabidopsis mitochondrial genome elicits cellular conditions for thermotolerance.

Shedge Vikas V Davila Jaime J Arrieta-Montiel Maria P MP Mohammed Saleem S Mackenzie Sally A SA

Plant physiology 20100205 4

Three nuclear genes involved in plant mitochondrial recombination surveillance have been previously identified. Simultaneous disruption of two of these genes, MutS Homolog1 (MSH1) and RECA3, results in extensive rearrangement of the mitochondrial genome and dramatic changes in plant growth. We have capitalized on these changes in mitochondrial genome organization to understand the role mitochondria play in plant cellular and developmental processes. Transcript profiling of the double mutants gro ...[more]

PMID: 20139171

Similar Datasets

Expression data from Arabipdosis msh1 recA3 double mutant under heat stress

Project description:In Arabidposis thaliana, the msh1 recA3 double mutant shows an extensive mitochondrial genome rearrangement and displays pronounced thermotolerance. We use microarray analysis to characterize genes and their response in the double mutant to a heat shock experiment in which plants were exposed to a temperature of 37 C for two hours.

2010-03-31 | GSE19603 | GEO

Transcription profiling of Arabidopsis wild type and msh1/recA3 double mutant plants

Project description:Double mutant msh1 and recA3

2009-07-01 | E-MEXP-1409 | biostudies-arrayexpress

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Project description:To identify biological function of HSFB transcriptional repressor, we subjected hsfb1 hsfb2b double mutant and wild-type Arabidopsis plant under heat condition (32 Celsius degree) and did microarray experiment. The microarray experiment under non-heated condition is described in GSE14702. We found expression of many heat-shock proteins (HSPs) is not fully enhanced in the double mutant when compared to wild-type plant. This data suggest that the inhibition of expression of HSPs in non-heated condition is important for fully heat-stress response in plant. hsfb1 hsfb2b double mutant vs. wild-type seedlings of Arabidopsis thaliana subjected under heat condition (32 Celsius degree). Biological replicates: 4 replicates.

2011-09-06 | E-GEOD-31883 | biostudies-arrayexpress

Expression data of QK mutant and wild type of Arabidopsis after heat shock

Project description:In order to study the importance of HSFA1 in thermotolerance in Arabidopsis, we generated the HSFA1a, b, d and e quadruple mutant (QK). QK is very sensitive to heat. Therefore, we used microarray to study how many genes regulated by HSFA1 after heat shock. Seven-day-old seedlings of Col-0, Ws and QK grown at 22oC on 0.5x MS plates containing 1% sucrose were incubated for 1 h at 37 °C. Subsequently, samples were collected for RNA extraction. The experiment was repeated, and two biological replicates were processed for analysis.

2010-12-23 | E-GEOD-26266 | biostudies-arrayexpress

Transcription profiling of yeast wild type BY4741, single deletion mutants delta-pbf1, delta-pbf2 and double mutant delta-pbf1 delta-pbf2 before and after heat shock

Project description:We used microarrays to detail the global program of gene expression underlying rRNA processing gene regulation during heat shock. PBF1 is YBL054W (TOD6) and PBF2 is YER088C (DOT6). Experiment Overall Design: S. cerevisiae cells were grown @ 25 ÂºC to O.D. 600 of 0.3~04, in triplicate. Half of the cells were collected at T0 and half of the cells were subjected to 25 ÂºC-->37 ÂºC heat shock and cells were collected after 20 min, as samples T20. Total RNAs were collected and used to perform expression microarray analysis on Affymetrix Yeast Array 2.0 platform. The changes of gene expression upon heat shock were analyzed for four strains, wild type BY4741, single deletion mutants delta-pbf1, delta-pbf2 and double mutant delta-pbf1 delta-pbf2.

2009-01-25 | E-GEOD-13684 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type and msh1 mutant plants

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Project description:apical meristem tissues from 4-week-old wild type, epiF3, MSH1 gene mutant including (mild-DR and dwarf-DR) plants were used in this experiment. For each genotype, the transcriptome was sequenced with 3 biological replicates.

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The role of (p)ppGpp in the heat shock response in B. subtilis

Project description:Bacterial and eukaryotic cells survive an otherwise lethal heat shock once they are primed by a mild pre-heat shock. Using B. subtilis as a model organism, we investigated transcriptional changes during thermotolerance development at 37 °C, 48 °C, 53 °C and 48/53 °C and observed a comprehensive upregulation of genes of the general and heat shock response and the downregulation of many translation-related genes. In addition, strains lacking (p)ppGpp (ppGpp0) or with elevated (p)ppGpp levels (ΔrelA) were analyzed at 37 °C and 48 °C, revealing broad and pleiotropic transcripional changes mediated by (p)ppGpp in the ΔrelA mutant. In addition, our data suggests a contribution of the (p)ppGpp-mediated stringent response in the heat-mediated transcripional down-regulation of ribosomal genes and additional processes.

2019-04-15 | GSE125467 | GEO

Yeast linker histone HHO1p effect on transcription before and after heat shock

Project description:DNA array expression analysis comparing RNA transcripts in wild type and hho1 strains before and after a heat-shock from 25 to 37 degrees. Keywords: expression, heat shock

2008-07-01 | GSE9095 | GEO

Nontargeted and targeted metabolomics measurements of abiotic stress responses in three-week-old Arabidopsis thaliana plants' rosette leaf tissue

Project description:Nontargeted and targeted metabolomics measurements of abiotic stress responses in three-week-old Arabidopsis thaliana plants' rosette leaf tissue for Col-0 wild type plants and double/triple knockout mutants of aquaporins (pip2;1 pip2;2 and pip2;1 pip2;2 pip2;4) treated with drought, heat at different air humidities, or combined drought-heat stress at different air humidities. This experiment contains FT-ICR-MS measurements for 103 Arabidopsis thaliana rosette leaf samples covering three genotypes under six different environmental conditions. The three genotypes comprise the Col-0 wildtype and two loss-of-function mutants of aquaporins, a pip2;1 pip2;2 double mutant and a pip2;1 pip2;2 pip2;4 triple mutant (respective AGI locus identifiers: AT3G53420, AT2G37170, AT5G60660). The six conditions include control condition (well-watered, 22 °C, 70% relative air humidity), drought stress (one week without watering), heat stress without changing the absolute humidity of the ambient air (6 hours at 33 °C, 37% relative air humidity), heat stress with supplemented air humidity to maintain a constant vapor pressure deficit before and during the heat episode (6 hours at 33 °C, 84% relative air humidity), and the combinations of drought pretreatment with each of the two heat stress variants (one week of drought followed by 6 hours of heat stress). Samples from all conditions were harvested at the same time (within 15 min starting at 5 pm). For validation, GC-TOF-MS measurements were done for two genotypes (wildtype, double mutant) and two conditions (drought, control) on partially overlapping samples.

2017-07-17 | MTBLS355 | MetaboLights

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