Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Connexin43 null vs wildtype neonatal mouse HEART


ABSTRACT: Cy3-labeled cDNA obtained from four pools of three hearts of neonatal C57BL Cx43 null mice were compared to Cy3-labeled cDNA obtained from four pools of three hearts of neonatal C57BL wildtype mice through Cy5-labeled sample reference prepared at once for the entire experiment from aorta, brain, heart, kidney, liver, lung, ovary/testicles, spleen, and stomach - equal amounts from adult male and female C57BL mice. Keywords = Cx32 null vs wildtype neonatal mouse heart

ORGANISM(S): Mus musculus

SUBMITTER: Dumitru Iacobas 

PROVIDER: E-GEOD-1961 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genes controlling multiple functional pathways are transcriptionally regulated in connexin43 null mouse heart.

Iacobas Dumitru A DA   Iacobas Sanda S   Li W E I WE   Zoidl Georg G   Dermietzel Rolf R   Spray David C DC  

Physiological genomics 20041207 3


We have used mouse 27k cDNA arrays to compare gene expression patterns in four sets of three hearts each of neonatal wild types and four sets of three hearts each of littermates lacking the major cardiac gap junction protein, connexin43 (Cx43). Each individual set of hearts was hybridized against aliquots of an RNA standard prepared from selected mouse tissues, allowing calculation of variability and coordination of gene expression among the samples from both genotypes. Overall variance of gene  ...[more]

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