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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Ceratitis capitata gene expression

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE19571: Ceratitis capitata gene expression related to sexual maturation in females GSE19572: Ceratitis capitata gene expression related to sexual maturation in males GSE19573: Ceratitis capitata gene expression related to mating status in mature females (mated vs virgin) GSE19608: Ceratitis capitata gene expression related to mating status in mature males (mated vs virgin) Refer to individual Series

ORGANISM(S): Ceratitis capitata

SUBMITTER: Ludvik Gomulski

PROVIDER: E-GEOD-19614 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Ceratitis capitata gene expression related to mating status in mature females (mated vs virgin)

Project description:This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata females This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata females. Samples where made from the heads of the females.

2011-12-25 | E-GEOD-19573 | biostudies-arrayexpress

Ceratitis capitata gene expression related to mating status in mature males (mated vs virgin)

Project description:This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata, males This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata, males. Samples where made from the heads of the males.

2011-12-25 | E-GEOD-19608 | biostudies-arrayexpress

Ceratitis capitata gene expression related to sexual maturation in females

Project description:This analysis compares gene expression between 1 day old and 4 day old virgin medfly, Ceratitis capitata females This analysis compares gene expression between 1 day old and 4 day old virgin medfly, Ceratitis capitata females. Samples where made from the heads of the females.

2011-12-25 | E-GEOD-19571 | biostudies-arrayexpress

Ceratitis capitata gene expression related to sexual maturation in males

Project description:This analysis compares gene expression between 1 day old and 4 day old virgin medfly, Ceratitis capitata, males This analysis compares gene expression between 1 day old and 4 day old virgin medfly, Ceratitis capitata males. Samples where made from the heads of the males.

2011-12-25 | E-GEOD-19572 | biostudies-arrayexpress

Ceratitis capitata gene expression related to sexual maturation in males

Project description:This analysis compares gene expression between 1 day old and 4 day old virgin medfly, Ceratitis capitata, males

2011-12-25 | GSE19572 | GEO

Ceratitis capitata gene expression related to sexual maturation in females

Project description:This analysis compares gene expression between 1 day old and 4 day old virgin medfly, Ceratitis capitata females

2011-12-25 | GSE19571 | GEO

Ceratitis capitata gene expression related to mating status in mature males (mated vs virgin)

Project description:This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata, males

2011-12-25 | GSE19608 | GEO

Ceratitis capitata gene expression related to mating status in mature females (mated vs virgin)

Project description:This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata females

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Honey bee heat-shock proteomics

Project description:Honey bee colonies were maintained in an apiary at the University of British Columbia. During the summer of 2018, 40 queens were reared from a single colony and half were allowed to open mate, while the other half were kept as virgins in plastic queen cages. Two weeks after emergence, the virgin queens were given two, eight-minute carbon dioxide treatments on 2 sequential days, then re-introduced to their nucleus colonies. This process stimulates virgin queens to begin laying71, and we conducted these treatments in order to minimize the physiological differences between virgin and mated queens. Virgin and mated queens were retrieved from their nucleus colonies and half of each (10) were subjected to heat-shock (42 ͦC, 2 h), and then maintained at 30 ͦC for 2 d. The other half were held only at 30 ͦC for 2 d. Four to six weeks after mating, the queens were anesthetized with carbon dioxide, beheaded, then their spermathecae (including the tracheal net) were removed with fine forceps. Both ovaries were also removed and weighed. During the same summer, 200 drones from a different colony in the same apiary were collected and maintained in the laboratory overnight at ambient temperature with excess syrup (50% sucrose). The next day, semen was harvested with glass capillaries according to the methods described above. Because many drones were not sexually mature, 60 semen samples (out of the 200 drones) were collected. Capillaries were placed in petri dishes and half (30) were heat-shocked as described above, then kept at 25 ͦC for 2 d. The other half were only kept at 25 ͦC for 2 d. Ten samples from each experimental group were used for sperm viability assays as described above.

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